RIP

产品目录编号

17-701

品牌系列

Upstate 

商名

• Upstate

• EZ-Magna        RIP

描述

EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation   Kit

概述

RNA-binding protein immunoprecipitation (RIP) is the   RNA analog of the more well-known ChIP application (chromatin   immunoprecipitation), which identifies DNA targets of DNA-binding proteins in   an in-vivo cellular context. RIP can be used to identify specific RNA   molecules (of many types) associated with specific nuclear or cytoplasmic   binding proteins. These experiments involve immunoprecipitation of   endogenously formed complexes of RNA-binding proteins and co-isolation of any   RNA species associated with that RNA-binding protein. Purification of these   RNA species allows interrogation and identification of mRNAs (and potentially   non-coding RNAs associated with them) and can be directly measured using down   stream applications including quantitative reverse transcription polymerase   chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing”   or 2nd-generation sequencing based platforms (RIP-Seq).
 
  Features & Benefits:
  -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune   complexes
  -RNAse inhibitors and RNAse-free reagents
  -Positive and negative controls

部件

Control Primers Magnetic Beads Protein A/G RIP Wash   Buffer RIP Lysis Buffer 0.5 M EDTA 10% SDS Salt Solution I Salt Solution II   Precipitate Enhancer Normal Mouse IgG Rabbit IgG Purified Protease Inhibitor   Cocktail 200X RNase Inhibitor Proteinase K (10 mg/mL) Nuclease free water   Positive Control Antibody (Anti-SNRNP70)

演示

Three boxes containing all necessary reagents to   perform 12 individual RNA-binding protein immunoprecipitation (RIP)   reactions.
  Includes positive control antibody and control primers.

应用

EZ-Magna RIP RNA-Binding Protein Immunoprecipitation   Kit containing all necessary reagents to perform 12 individual RNA-binding   protein immunoprecipitation (RIP) reactions.

主要应用

• RNA Binding        Protein Immunoprecipitation (RIP)

使用声明

• Unless        otherwise stated in our catalog or other company documentation        accompanying the product(s), our products are intended for research use        only and are not to be used for any other purpose, which includes but is        not limited to, unauthorized commercial uses, in vitro diagnostic uses,        ex vivo or in vivo therapeutic uses or any type of consumption or        application to humans or animals.

存储条件

Upon receipt, store components at the temperatures   indicated on the labels. Kit components are stable for 6 months from date of   shipment when stored as directed.

材料尺寸

12 assays

材料包装

RIP Kit capacity: 12 RNA-binding protein   immunoprecipitation assays